Recombinant human NXT1 protein

NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein) and CRM1 (required for chromosome region maintenance) dependent pathways. It is found to stimulate the export of u1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. This protien heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. Recombinant human NXT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

NTF2-related export protein 1, MTR2, P15

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by BCA assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

NXT1, NTF2-related export protein 1, MTR2, P15, ATGP1045-10 µg, ATGP1045-20 µg, ATGP1045-50 µg, ATGP1045-100 µg, ATGP1045-250 µg, ATGP1045-500 µg, ATGP1045-1 mg, ATGP1045-10, ATGP1045-20, ATGP1045-50, ATGP1045-100, ATGP1045-250, ATGP1045-500, ATGP1045-1

References & Citations

Black B.E., et al. (1999) Mol. Cell. Biol. 19:8616-8624; ; Ossareh-Nazari B., et al. (2000) Mol. Cell. Biol. 20:4562-4571

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_037380

Uniprot Accession Number

Q9UKK6

Species

Human

AA Sequence

MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN AVSGQESLSE FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK FEGNKQRDFN QNFILTAQAS PSNTVWKIAS DCFRFQDWAS