Recombinant human SNTN protein
SNTN, also known as sentan, belongs to the S-100 family. SNTN is shown to localize exclusively to the bridging structure between the cell membrane and peripheral singlet microtubules, which specifically exists in the narrowed distal portion of cilia. Exogenously expressed sentan showed affinity for the membrane protrusions, and a protein-lipid binding assay revealed that sentan bound to phosphatidylserine. These findings suggest that sentan is the first molecular component of the ciliary tip to bridge the cell membrane and peripheral singlet microtubules, making the distal portion of the cilia narrow and stiff to allow for better airway clearance or ovum transport. Recombinant human SNTN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Sentan, S100A1L, S100AL
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
18.6 kDa (167aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Muzny D.M., et al. (2006) Nature. 440:1194-1198; ; Kubo A., et al. (2008) Mol. Biol. Cell 19:5338-5346
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001074006
Uniprot Accession Number
A6NMZ2
Species
Human
AA Sequence
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