Recombinant human CCL4L1/MIP-1 beta protein
CCL4L1 (C-C motif chemokine 4-like) belongs to intercrine beta (chemokine CC) family. This protein is similar to CCL4 which inhibits HIV replication in peripheral blood monocytes that express CCR5. Recombinant human CCL4L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
10.5 kDa (94aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Howard O.M.Z., et al. (2004) Biochem. Biophys. Res. Commun. 320:927-931; ; Colobran R, et al. (2005) J Immunol. 174 (9) :5655-64.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_996890.1
Uniprot Accession Number
Q8NHW4
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAPMGS DPPTACCFSY TARKLPRNFV VDYYETSSLC SQPAVVFQTK RGKQVCADPS ESWVQEYVYD LELN
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