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Recombinant human CCL4L1/MIP-1 beta protein

CCL4L1 (C-C motif chemokine 4-like) belongs to intercrine beta (chemokine CC) family. This protein is similar to CCL4 which inhibits HIV replication in peripheral blood monocytes that express CCR5. Recombinant human CCL4L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

C-C motif chemokine ligand 4 like 1, Lymphocyte activation gene 1 protein, LAG-1, Macrophage inflammatory protein 1-beta, MIP-1-beta, Monocyte adherence-induced protein 5-alpha, Small-inducible cytokine A4-like, SCYA4L, AT744.2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

10.5 kDa (94aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CCL4L1, MIP1 b, C-C motif chemokine 4-like, AT744.2, CCL4L, CCL4L2, LAG-1, LAG1, SCYA4L, ATGP1042-10 µg, ATGP1042-20 µg, ATGP1042-50 µg, ATGP1042-100 µg, ATGP1042-250 µg, ATGP1042-500 µg, ATGP1042-1 mg, ATGP1042-10, ATGP1042-20, ATGP1042-50, ATGP1042-100, ATGP1042-250, ATGP1042-500, ATGP1042-1

References & Citations

Howard O.M.Z., et al. (2004) Biochem. Biophys. Res. Commun. 320:927-931; ; Colobran R, et al. (2005) J Immunol. 174 (9) :5655-64.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_996890.1

Uniprot Accession Number

Q8NHW4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAPMGS DPPTACCFSY TARKLPRNFV VDYYETSSLC SQPAVVFQTK RGKQVCADPS ESWVQEYVYD LELN

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