Recombinant e.coli cysH protein

CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3', 5'-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3'-phosphoadenylyl sulfate and thioredoxin. Recombinant E. coli cysH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphoadenosine phosphosulfate reductase

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.1 kDa (264aa) confirmed by MALDI-TOF

Additionnal Information

CysH, Phosphoadenosine phosphosulfate reductase, ATGP1041-10 µg, ATGP1041-20 µg, ATGP1041-50 µg, ATGP1041-100 µg, ATGP1041-250 µg, ATGP1041-500 µg, ATGP1041-1 mg, ATGP1041-10, ATGP1041-20, ATGP1041-50, ATGP1041-100, ATGP1041-250, ATGP1041-500, ATGP1041-1

References & Citations

Berendt u., et al. (1995) Eur. J. Biochem. 233 (1) : 347-56.; ; Krone F.A., et al. (1990) FEBS Lett. 260:6-9

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_417242

Uniprot Accession Number

P17854

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG