Recombinant e.coli grxB protein

GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.5 kDa (235aa) confirmed by MALDI-TOF

Additionnal Information

GrxB, glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2, ATGP1040-10 µg, ATGP1040-20 µg, ATGP1040-50 µg, ATGP1040-100 µg, ATGP1040-250 µg, ATGP1040-500 µg, ATGP1040-1 mg, ATGP1040-10, ATGP1040-20, ATGP1040-50, ATGP1040-100, ATGP1040-250, ATGP1040-500, ATGP1040-1

References & Citations

Holmgren A, et al. (2004) Antioxid. Redox. Signal. 6 (1) : 63-74.; ; Lopez-Maury L, et al. (2009) J Bacteriol. 191 (11) :3534-43.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_415582

Uniprot Accession Number

P0AC59

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI