Recombinant human LC3B/MAP1LC3B protein

Microtubule-associated proteins 1A/1B light chain 3 beta 2, also as known as MAP1LC3B2, belongs to the MAP1LC3 family. MAP1LC3B2 is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. This protein probably involved in formation of autophagosomal vacuoles (autophagosomes) . It is expressed primarily in heart, testis, brain and skeletal muscle and testis. Recombinant human MAP1LC3B2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Microtubule-associated proteins 1A/1B light chain 3B, Microtubule associated protein 1 light chain 3 beta, MAP1ALC3, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B, ATG8F

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.2 kDa (140aa) confirmed by MALDI-TOF

Additionnal Information

MAP1LC3B2, Microtubule-associated proteins 1A/1B light chain 3 beta 2, ATG8G, ATGP1039-10 µg, ATGP1039-20 µg, ATGP1039-50 µg, ATGP1039-100 µg, ATGP1039-250 µg, ATGP1039-500 µg, ATGP1039-1 mg, ATGP1039-10, ATGP1039-20, ATGP1039-50, ATGP1039-100, ATGP1039-250, ATGP1039-500, ATGP1039-1

References & Citations

He H., et al. (2003) J. Biol. Chem. 278:29278-29287; ; Seidenbecher C.I., et al. (2004) J. Mol. Biol. 336:957-970

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_001078950

Uniprot Accession Number

A6NCE7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVCASQETFG