Recombinant human Otubain-2/OTUB2 protein

OTuB2 (ubiquitin thioesterase OTuB2) belongs to the peptidase C65 family. OTuB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Recombinant human OTuB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin thioesterase OTuB2, OTB2, OTu2, TimntanOTUB2 aUgitin thioesterase 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.3 kDa (254aa) confirmed by MALDI-TOF

Additionnal Information

Otubain2, ubiquitin thioesterase OTuB2, OTB2, OTu2, ATGP1038-10 µg, ATGP1038-20 µg, ATGP1038-50 µg, ATGP1038-100 µg, ATGP1038-250 µg, ATGP1038-500 µg, ATGP1038-1 mg, ATGP1038-10, ATGP1038-20, ATGP1038-50, ATGP1038-100, ATGP1038-250, ATGP1038-500, ATGP1038-1

References & Citations

Balakirev MY, et al. (2003) EMBO Rep 4 (5) : 517-22.; ; Edelmann M.J., et al. (2009) Biochem. J. 418:379-390

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Ubiquitination

NCBI Accession Number

NP_075601

Uniprot Accession Number

Q96DC9

Species

Human

AA Sequence

MSETSFNLIS EKCDILSILR DHPENRIYRR KIEELSKRFT AIRKTKGDGN CFYRALGYSY LESLLGKSRE IFKFKERVLQ TPNDLLAAGF EEHKFRNFFN AFYSVVELVE KDGSVSSLLK VFNDQSASDH IVQFLRLLTS AFIRNRADFF RHFIDEEMDI KDFCTHEVEP MATECDHIQI TALSQALSIA LQVEYVDEMD TALNHHVFPE AATPSVYLLY KTSHYNILYA ADKH