Recombinant e.coli sodA protein

Superoxide dismutase, Mn, also known as sodA, is a member of the iron/manganese superoxide dismutase family. SodA destroys radicals which are normally produced within the cells and which are toxic to biological systems. It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. Recombinant E. coli sodA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 300 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Product Name Alternative

Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 300unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Molecular Weight

25.2 kDa (226aa) confirmed by MALDI-TOF

Additionnal Information

SodA, Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD, ATGP1037-10 µg, ATGP1037-20 µg, ATGP1037-50 µg, ATGP1037-100 µg, ATGP1037-250 µg, ATGP1037-500 µg, ATGP1037-1 mg, ATGP1037-10, ATGP1037-20, ATGP1037-50, ATGP1037-100, ATGP1037-250, ATGP1037-500, ATGP1037-1

References & Citations

Barrister J V., et al. (1987) Crit Rev Biochem. 22:11-180.; ; ujika I., et al. (1991) Acta Histochem Cytochem. 24:489-495.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_418344

Uniprot Accession Number

P00448

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK