Recombinant e.coli msrA protein
Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Recombinant E. coli msrA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.4 kDa (232aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kuschel L., et al. (1999) FEBS Letts.1:17-21.; ; Gabbita S., et al. (1999) J Neurochem. 4:1660-1666.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
NP_418640
Uniprot Accession Number
P0A744
Species
E.coli
AA Sequence
Frequently Asked Questions
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