Recombinant e.coli msrA protein

Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Recombinant E. coli msrA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.4 kDa (232aa) confirmed by MALDI-TOF

Additionnal Information

MsrA, Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase, ATGP1036-10 µg, ATGP1036-20 µg, ATGP1036-50 µg, ATGP1036-100 µg, ATGP1036-250 µg, ATGP1036-500 µg, ATGP1036-1 mg, ATGP1036-10, ATGP1036-20, ATGP1036-50, ATGP1036-100, ATGP1036-250, ATGP1036-500, ATGP1036-1

References & Citations

Kuschel L., et al. (1999) FEBS Letts.1:17-21.; ; Gabbita S., et al. (1999) J Neurochem. 4:1660-1666.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_418640

Uniprot Accession Number

P0A744

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA