Recombinant human Galectin-10/LGALS10 protein

Eosinophil lysophospholipase (CLC), also known as Galectin 10, act on biological membranes to regulate the multifunctional lysophospholipids. CLC is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias. Recombinant human CLC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Lysolecithin acylhydrolase, LPPL, LGALS10A, LGALS10, Lectin galactoside-binding soluble, Galectin 10, Gal-10, Eosinophil lysophospholipase, CLC, Charcot-Leyden crystal protein, 10LGALS10A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.6 kDa (162aa) confirmed by MALDI-TOF

Additionnal Information

Lysolecithin acylhydrolase, LPPL, LGALS10A, LGALS10, Lectin galactoside-binding soluble, Galectin-10, Galectin10, Galectin 10, Gal-10, Gal10, Gal 10, Eosinophil lysophospholipase, CLC, Charcot-Leyden crystal protein, Charcot Leyden crystal protein, 10LGALS10A, ATGP1034-10 µg, ATGP1034-20 µg, ATGP1034-50 µg, ATGP1034-100 µg, ATGP1034-250 µg, ATGP1034-500 µg, ATGP1034-1 mg, ATGP1034-010, ATGP1034-020, ATGP1034-050, ATGP1034-100, ATGP1034-250, ATGP1034-500, ATGP1034-01M

References & Citations

Gieich G J., et al. (1976) J Clin Invest. 57:633-640.; ; Swaminathan G J., et al. (1999) Biochemistry 38:13837-13843.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001819.2

Uniprot Accession Number

Q05315

Species

Human

AA Sequence

MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI VFHFQVCFGR RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR