Recombinant human CIRBP protein

Cold inducible RNA-binding protein, also known as CIRBP, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. It is localizes to the nucleus and contains one RRM (RNA recognition motif) domain. Expressed ubiquitously, CIRBP is thought to play an essential role in the suppression of cellular proliferation in response to uV irradiation or extreme cold. Recombinant human CIRBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cold inducible RNA-binding protein, CIRP, A18HNRNP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.8 kDa (192aa) confirmed by MALDI-TOF

Additionnal Information

CIRBP, ATGP1033-10 µg, ATGP1033-20 µg, ATGP1033-50 µg, ATGP1033-100 µg, ATGP1033-250 µg, ATGP1033-500 µg, ATGP1033-1 mg, ATGP1033-10, ATGP1033-20, ATGP1033-50, ATGP1033-100, ATGP1033-250, ATGP1033-500, ATGP1033-1

References & Citations

Sheikh MS., et al. (1997) J Biol Chem. 272 (42) :26720-6; ; Nishiyama H., et al. (1997) Gene. 204:115-120.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001271

Uniprot Accession Number

Q14011

Species

Human

AA Sequence

MASDEGKLFV GGLSFDTNEQ SLEQVFSKYG QISEVVVVKD RETQRSRGFG FVTFENIDDA KDAMMAMNGK SVDGRQIRVD QAGKSSDNRS RGYRGGSAGG RGFFRGGRGR GRGFSRGGGD RGYGGNRFES RSGGYGGSRD YYSSRSQSGG YSDRSSGGSY RDSYDSYATH NE