Recombinant human Tau 412 (1N4R) /MAPT protein

Tau, also known as microtubule-associated protein tau (MAPT), is a protein that stabilizes microtubules. It is abundant in neurons in the central nervous system and is less common elsewhere. When this protein is defective, and no longer stabilizes microtubules property, it can result in dementias, such as Alzheimer's disease. Recombinant human Tau protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Microtubule-associated protein tau isoform 5, tau-E, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, G protein beta1/gamma2 subunit-interacting factor 1, protein phosphatase 1 regulatory subunit 103, MTBT1, PPND, FTDP-17, TAU, MSTD, MTBT2, FLJ31424, MGC138549, PPP1R103, tau-40, DDPAC, MAPTL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

45.1 kDa (432aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Tau, Microtubule-associated protein tau isoform 5, MAPT, MAPTL, MTBT1, TAu, PHF-tau, ATGP1030-10 µg, ATGP1030-20 µg, ATGP1030-50 µg, ATGP1030-100 µg, ATGP1030-250 µg, ATGP1030-500 µg, ATGP1030-1 mg, ATGP1030-10, ATGP1030-20, ATGP1030-50, ATGP1030-100, ATGP1030-250, ATGP1030-500, ATGP1030-1

References & Citations

Cross D., et al. (1993), J Cell Sci. 105 : 51-60.; ; Lubke u., et al. (1994), Am. J. Pathol. 145: 175-188

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001116539

Uniprot Accession Number

P10636

Species

Human

AA Sequence

MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEAEEAGIG DTPSLEDEAA GHVTQARMVS KSKDGTGSDD KKAKGADGKT KIATPRGAAP PGQKGQANAT RIPAKTPPAP KTPPSSGEPP KSGDRSGYSS PGSPGTPGSR SRTPSLPTPP TREPKKVAVV RTPPKSPSSA KSRLQTAPVP MPDLKNVKSK IGSTENLKHQ PGGGKVQIIN KKLDLSNVQS KCGSKDNIKH VPGGGSVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV KSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA DEVSASLAKQ GL