Recombinant human NUDT10 protein
NuDT10, also known as APS2 or DIPP3alpha, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8. 5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates. Recombinant human NuDT10 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
19.5 kDa (172aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hidaka K. et al. (2002) J. Biol. Chem. 277: 32730-32738; ; Hua L.V. et al. (2003) Biochem. J. 373: 81-89
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_694853
Uniprot Accession Number
Q8NFP7
Species
Human
Frequently Asked Questions
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