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Recombinant human NUDT10 protein

NuDT10, also known as APS2 or DIPP3alpha, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8. 5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates. Recombinant human NuDT10 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Nudix hydrolase 10, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, hDIPP3alpha, Nucleoside diphosphate-linked moiety X motif 10, Nudix motif 10, hAps2, APS2, DIPP3A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.5 kDa (172aa) confirmed by MALDI-TOF

Additionnal Information

NuDT10, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, APS2, DIPP3a, hDIPP3alpha, ATGP1029-10 µg, ATGP1029-20 µg, ATGP1029-50 µg, ATGP1029-100 µg, ATㅁNudix hydrolase 10, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, hDIPP3alpha, Nucleoside diphosphate-linked moiety X motif 10, Nudix motif 10, hAps2, APS2, DIPP3A, NuDT10, ATGP1029-10 µg, ATGP1029-20 µg, ATGP1029-50 µg, ATGP1029-100 µg, ATGP1029-250 µg, ATGP1029-500 µg, ATGP1029-1 mg, ATGP1029-010, ATGP1029-020, ATGP1029-050, ATGP1029-100, ATGP1029-250, ATGP1029-500, ATGP1029-01M GP1029-250 µg, ATGP1029-500 µg, ATGP1029-1 mg, ATGP1029-10, ATGP1029-20, ATGP1029-50, ATGP1029-100, ATGP1029-250, ATGP1029-500, ATGP1029-1

References & Citations

Hidaka K. et al. (2002) J. Biol. Chem. 277: 32730-32738; ; Hua L.V. et al. (2003) Biochem. J. 373: 81-89

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_694853

Uniprot Accession Number

Q8NFP7

Species

Human

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