Recombinant human NPL protein
NPL, also known as N-acetylneuraminate lyase, is an enzyme that catalyzes the chemical reaction. (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate) This protein belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. It participates in amino sugars metabolism. Recombinant human NPL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
N-acetylneuraminate lyase, c112, C1orf13, DHDPS1, MGC149582, MGC61869, NPL1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.3 kDa (340aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Schauer R. et al. (1982) Adv Carbohydr Chem Biochem. 40:131-234.; ; COMB DG. et al. (1960) J Biol Chem. 235:2529-2537
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_110396
Uniprot Accession Number
Q9BXD5
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
