Recombinant human NPL protein

NPL, also known as N-acetylneuraminate lyase, is an enzyme that catalyzes the chemical reaction. (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate) This protein belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. It participates in amino sugars metabolism. Recombinant human NPL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

N-acetylneuraminate lyase, c112, C1orf13, DHDPS1, MGC149582, MGC61869, NPL1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.3 kDa (340aa) confirmed by MALDI-TOF

Additionnal Information

NPL, N-acetylneuraminate lyase, c112, C1orf13, DHDPS1, MGC149582, MGC61869, NPL1, ATGP1028-10 µg, ATGP1028-20 µg, ATGP1028-50 µg, ATGP1028-100 µg, ATGP1028-250 µg, ATGP1028-500 µg, ATGP1028-1 mg, ATGP1028-10, ATGP1028-20, ATGP1028-50, ATGP1028-100, ATGP1028-250, ATGP1028-500, ATGP1028-1

References & Citations

Schauer R. et al. (1982) Adv Carbohydr Chem Biochem. 40:131-234.; ; COMB DG. et al. (1960) J Biol Chem. 235:2529-2537

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_110396

Uniprot Accession Number

Q9BXD5

Species

Human

AA Sequence

MAFPKKKLQG LVAATITPMT ENGEINFSVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSV SERRQVAEEW VTKGKDKLDQ VIIHVGALSL KESQELAQHA AEIGADGIAV IAPFFLKPWT KDILINFLKE VAAAAPALPF YYYHIPALTG VKIRAEELLD GILDKIPTFQ GLKFSDTDLL DFGQCVDQNR QQQFAFLFGV DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDFS LALNYQFCIQ RFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDSAEAKL KSLDFLSFTD LKDGNLEAGS