Recombinant e.coli msrB protein

MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E. coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Methionine sulfoxide reductase B, ECK1776, JW1767, yeaA

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.6 kDa (157aa) confirmed by MALDI-TOF

Additionnal Information

MsrB, Methionine sulfoxide reductase B, ECK1776, JW1767, yeaA, ATGP1026-10 µg, ATGP1026-20 µg, ATGP1026-50 µg, ATGP1026-100 µg, ATGP1026-250 µg, ATGP1026-500 µg, ATGP1026-1 mg, ATGP1026-10, ATGP1026-20, ATGP1026-50, ATGP1026-100, ATGP1026-250, ATGP1026-500, ATGP1026-1

References & Citations

Zheung D. et al. (2003) J. Biomol. NMR. 27:183-184; ; Weissbach H. et al. (2002) Arch.Biochem.Biophys. 397: 172-178

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_416292

Uniprot Accession Number

P0A746

Species

E.coli

AA Sequence

MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING