Recombinant e.coli Methionine Aminopeptidase/METAP protein

Methionine aminopeptidases, also designated peptidase M proteins, are members of the M24 family of proteins. This protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of map contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant E. coli map protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Methionine aminopeptidase., ECK0166, JW0163, pepM

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.5 kDa (284aa) confirmed by MALDI-TOF

Additionnal Information

Methionine Aminopeptidase, METAP, Methionine aminopeptidase., ECK0166, JW0163, pepM, ATGP1025-10 µg, ATGP1025-20 µg, ATGP1025-50 µg, ATGP1025-100 µg, ATGP1025-250 µg, ATGP1025-500 µg, ATGP1025-1 mg, ATGP1025-10, ATGP1025-20, ATGP1025-50, ATGP1025-100, ATGP1025-250, ATGP1025-500, ATGP1025-1

References & Citations

Bernier SG., et al. (2005) J Cell Biochem. 95 (6) :1191-203.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_414710

Uniprot Accession Number

P0AE18

Species

E.coli

AA Sequence

MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE