Recombinant human eIF-2B alpha/EIF2B1 protein

EIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Phosphorylation of eIF2 inhibits GEF activity of EIF2B, an inhibition that requires the eIF2B1 subunit. Defects in eIF2B1 are a cause of leukoencephalopathy with vanishing white matter (VWM), a brain disease that is characterized by head trauma and motor deterioration. Recombinant human EIF2B1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 2B subunit alpha, Eukaryotic translation initiation factor 2B subunit 1 alpha 26kDa, EIF-2Balpha, EIF-2B, EIF2BA, EIF2Balpha, Translation initiation factor eIF-2B subunit alpha, eIF-2B GDP-GTP exchange factor subunit alpha

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.8 kDa (325aa) confirmed by MALDI-TOF

Additionnal Information

EIF2B1, Eukaryotic translation initiation factor 2B subunit 1 alpha 26kD, Eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kD, EIF-2B, EIF-2Balpha, EIF2B, EIF2BA, MGC117409, MGC125868, MGC125869, ATGP1024-10 µg, ATGP1024-20 µg, ATGP1024-50 µg, ATGP1024-100 µg, ATGP1024-250 µg, ATGP1024-500 µg, ATGP1024-1 mg, ATGP1024-10, ATGP1024-20, ATGP1024-50, ATGP1024-100, ATGP1024-250, ATGP1024-500, ATGP1024-1

References & Citations

Van der Knaap M.S. et al. (2002) Ann. Neurol 51: 264-270 ; ; Mohammad-Qureshi S.S. et al. (2007) Mol. Cell. Biol. 27: 5225-5234.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Scientific Category

Development

NCBI Accession Number

NP_001405.1

Uniprot Accession Number

Q14232

Species

Human

AA Sequence

MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVD SSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIK DGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GKKMAKALCH LNVPVTVVLD AAVGYIMEKA DLVIVGAEGV VENGGIINKI GTNQMAVCAK AQNKPFYVVA ESFKFVRLFP LNQQDVPDKF KYKADTLKVA QTGQDLKEEH PWVDYTAPSL ITLLFTDLGV LTPSAVSDEL IKLYL