Recombinant human eIF-2B alpha/EIF2B1 protein
EIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Phosphorylation of eIF2 inhibits GEF activity of EIF2B, an inhibition that requires the eIF2B1 subunit. Defects in eIF2B1 are a cause of leukoencephalopathy with vanishing white matter (VWM), a brain disease that is characterized by head trauma and motor deterioration. Recombinant human EIF2B1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.8 kDa (325aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Van der Knaap M.S. et al. (2002) Ann. Neurol 51: 264-270 ; ; Mohammad-Qureshi S.S. et al. (2007) Mol. Cell. Biol. 27: 5225-5234.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
Scientific Category
Development
NCBI Accession Number
NP_001405.1
Uniprot Accession Number
Q14232
Species
Human
AA Sequence
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