Recombinant e.coli hchA protein

HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hsp31 molecular chaperone, ECK1963, JW1950, yedu, yzzC

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.3 kDa (303aa) confirmed by MALDI-TOF

Additionnal Information

HchA, Hsp31 molecular chaperone, ECK1963, JW1950, yedu, yzzC, ATGP1023-10 µg, ATGP1023-20 µg, ATGP1023-50 µg, ATGP1023-100 µg, ATGP1023-250 µg, ATGP1023-500 µg, ATGP1023-1 mg, ATGP1023-10, ATGP1023-20, ATGP1023-50, ATGP1023-100, ATGP1023-250, ATGP1023-500, ATGP1023-1

References & Citations

Malki A. et al. (2003) Biochem. Biophys. Res. Commun. 301:430-436.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_416476

Uniprot Accession Number

P31658

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG