Recombinant e.coli deoC protein

DeoC is a member of the deoC/fbaB aldolase protein family. The systematic name of this enzyme class is 2-deoxyribose-5-phosphate aldolase, which cleave carbon-carbon bonds. This protein is Involved in the carbohydrate degradation pathway, catalyzes the conversion of 2-deoxy-D-ribose 5-phosphate to D-glyceraldehyde 3-phosphate and an acetyldehyde. Recombinant E. coli deoC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Deoxyribose-phosphate aldolase, dra, ECK4373, JW4344, thyR, DERA

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.9 kDa (279aa) confirmed by MALDI-TOF

Additionnal Information

DeoC, Deoxyribose-phosphate aldolase, dra, ECK4373, JW4344, thyR, DERA, ATGP1021-10 µg, ATGP1021-20 µg, ATGP1021-50 µg, ATGP1021-100 µg, ATGP1021-250 µg, ATGP1021-500 µg, ATGP1021-1 mg, ATGP1021-10, ATGP1021-20, ATGP1021-50, ATGP1021-100, ATGP1021-250, ATGP1021-500, ATGP1021-1

References & Citations

Sgarrella F., et al. (1997) Comp Biochem Physiol B Biochem Mol Biol. 117 (2) :253-7. ; ; Horinouchi N., et al. (2006) Biosci Biotechnol Biochem. 70 (6) :1371-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418798

Uniprot Accession Number

P0A6L0

Species

E.coli

AA Sequence

MTDLKASSLR ALKLMDLTTL NDDDTDEKVI ALCHQAKTPV GNTAAICIYP RFIPIARKTL KEQGTPEIRI ATVTNFPHGN DDIDIALAET RAAIAYGADE VDVVFPYRAL MAGNEQVGFD LVKACKEACA AANVLLKVII ETGELKDEAL IRKASEISIK AGADFIKTST GKVAVNATPE SARIMMEVIR DMGVEKTVGF KPAGGVRTAE DAQKYLAIAD ELFGADWADA RHYRFGASSL LASLLKALGH GDGKSASSY