Recombinant human ALKBH2 protein
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and alpha-ketoglutarate for enzymatic activity. Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, ABH2, Oxy DC1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
31.4 kDa (281aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Rinqvoll J., et al. (2008) Cancer Res. 68:4142-4149.; ; Lee D H., et al. (2005) J Biol Chem. 280:39448-39459.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001138847.1
Uniprot Accession Number
Q6NS38
Species
Human
AA Sequence
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