Recombinant human ALKBH2 protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and alpha-ketoglutarate for enzymatic activity. Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, ABH2, Oxy DC1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.4 kDa (281aa) confirmed by MALDI-TOF

Additionnal Information

ALKBH2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, ABH2, Oxy DC1, ATGP1016-10 µg, ATGP1016-20 µg, ATGP1016-50 µg, ATGP1016-100 µg, ATGP1016-250 µg, ATGP1016-500 µg, ATGP1016-1 mg, ATGP1016-10, ATGP1016-20, ATGP1016-50, ATGP1016-100, ATGP1016-250, ATGP1016-500, ATGP1016-1

References & Citations

Rinqvoll J., et al. (2008) Cancer Res. 68:4142-4149.; ; Lee D H., et al. (2005) J Biol Chem. 280:39448-39459.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001138847.1

Uniprot Accession Number

Q6NS38

Species

Human

AA Sequence

MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K