Recombinant human FARS2 protein

Phenylalanyl-tRNA synthetase 2, mitochondrial (PheRS), also known as FARS2, belongs to the class II aminoacyl- tRNA synthetase family. FARS2 is a mitochondrial matrix protein. Functioning as a monomer, FARS2 catalyzes the ATP-dependent conversion of L-phenylalanine and tRNA (Phe) to L-phenlalanyltRNA (Phe), an event that is crucial for proper translation and protein expression. Recombinant human FARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phenylalanyl-tRNA synthetase 2 mitochondrial, Phenylalanyl-tRNA synthetase 2, mitochondrial, dJ520B18.2, FARS1, HSPC320, PheRS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

50.6 kDa (436aa) confirmed by MALDI-TOF

Additionnal Information

FARS2, ATGP1015-10 µg, ATGP1015-20 µg, ATGP1015-50 µg, ATGP1015-100 µg, ATGP1015-250 µg, ATGP1015-500 µg, ATGP1015-1 mg, ATGP1015-10, ATGP1015-20, ATGP1015-50, ATGP1015-100, ATGP1015-250, ATGP1015-500, ATGP1015-1

References & Citations

Sasaki H M., et al. (2006) Proc Natl Acad Sci uSA. 103:14744-14749.; ; Bullard J M., et al. (1999) J Mol Biol. 288:567-577.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_006558.1

Uniprot Accession Number

O95363

Species

Human

AA Sequence

PAAECATQR APGSVVELLG KSYPQDDHSN LTRKVLTRVG RNLHNQQHHP LWLIKERVKE HFYKQYVGRF GTPLFSVYDN LSPVVTTWQN FDSLLIPADH PSRKKGDNYY LNRTHMLRAH TSAHQWDLLH AGLDAFLVVG DVYRRDQIDS QHYPIFHQLE AVRLFSKHEL FAGIKDGESL QLFEQSSRSA HKQETHTMEA VKLVEFDLKQ TLTRLMAHLF GDELEIRWVD CYFPFTHPSF EMEINFHGEW LEVLGCGVME QQLVNSAGAQ DRIGWAFGLG LERLAMILYD IPDIRLFWCE DERFLKQFCV SNINQKVKFQ PLSKYPAVIN DISFWLPSEN YAENDFYDLV RTIGGDLVEK VDLIDKFVHP KTHKTSHCYR ITYRHMERTL SQREVRHIHQ ALQEAAVQLL GVEGRF