Recombinant human FARS2 protein
Phenylalanyl-tRNA synthetase 2, mitochondrial (PheRS), also known as FARS2, belongs to the class II aminoacyl- tRNA synthetase family. FARS2 is a mitochondrial matrix protein. Functioning as a monomer, FARS2 catalyzes the ATP-dependent conversion of L-phenylalanine and tRNA (Phe) to L-phenlalanyltRNA (Phe), an event that is crucial for proper translation and protein expression. Recombinant human FARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Phenylalanyl-tRNA synthetase 2 mitochondrial, Phenylalanyl-tRNA synthetase 2, mitochondrial, dJ520B18.2, FARS1, HSPC320, PheRS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
50.6 kDa (436aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sasaki H M., et al. (2006) Proc Natl Acad Sci uSA. 103:14744-14749.; ; Bullard J M., et al. (1999) J Mol Biol. 288:567-577.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_006558.1
Uniprot Accession Number
O95363
Species
Human
AA Sequence
Frequently Asked Questions
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