Recombinant human CTDSP1 protein

Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recombinant human CTDSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1, Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, NLIIF, SCP1, NIF3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.2 kDa (280aa) confirmed by MALDI-TOF

Additionnal Information

CTDSP1, Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1, Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, NLIIF, SCP1, NIF3, ATGP1013-10 µg, ATGP1013-20 µg, ATGP1013-50 µg, ATGP1013-100 µg, ATGP1013-250 µg, ATGP1013-500 µg, ATGP1013-1 mg, ATGP1013-10, ATGP1013-20, ATGP1013-50, ATGP1013-100, ATGP1013-250, ATGP1013-500, ATGP1013-1

References & Citations

Maiello B., et al. (2001) Front Biosci. 6: 1358-1368.; ; Palancade B., et al. (2004) J Mol Biol. 335:415-424.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_872580

Uniprot Accession Number

Q9GZU7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS