Recombinant human SPOP protein
SPOP (speckle-type POZ protein), also known as TEF2, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF homology) domain and one C-terminal BTB/POZ domain. This protein inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. Through an interaction with CuL-3, SPOP is involved in ubiquitinylation and protein degradation. Recombinant human SPOP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Speckle-type POZ protein, TEF2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
44.3 kDa (394aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kwon J.E., et al. (2006) J. Biol. Chem. 281:12664-12672; ; Furukawa M., et al. (2003) Nat. Cell Biol. 5:1001-1007
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA
Scientific Category
Ubiquitination
NCBI Accession Number
NP_001007231
Uniprot Accession Number
O43791
Species
Human
AA Sequence
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