Recombinant human SPOP protein

SPOP (speckle-type POZ protein), also known as TEF2, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF homology) domain and one C-terminal BTB/POZ domain. This protein inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. Through an interaction with CuL-3, SPOP is involved in ubiquitinylation and protein degradation. Recombinant human SPOP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Speckle-type POZ protein, TEF2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.3 kDa (394aa) confirmed by MALDI-TOF

Additionnal Information

SPOP, Speckle-type POZ protein, TEF2, ATGP1009-10 µg, ATGP1009-20 µg, ATGP1009-50 µg, ATGP1009-100 µg, ATGP1009-250 µg, ATGP1009-500 µg, ATGP1009-1 mg, ATGP1009-10, ATGP1009-20, ATGP1009-50, ATGP1009-100, ATGP1009-250, ATGP1009-500, ATGP1009-1

References & Citations

Kwon J.E., et al. (2006) J. Biol. Chem. 281:12664-12672; ; Furukawa M., et al. (2003) Nat. Cell Biol. 5:1001-1007

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA

Scientific Category

Ubiquitination

NCBI Accession Number

NP_001007231

Uniprot Accession Number

O43791

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ CPFLGPPRKR LKQS