Recombinant human SEC13 protein
SEC13 belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. This protein is Interacts with SEC31A and SEC31B. SEC31A was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function. Recombinant human SEC13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Protein SEC13 homolog, D3S1231E, SEC13L1, SEC13R
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.7 kDa (342aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Stankewich M.C., et al. (2006) J. Cell Sci. 119:958-969; ; Yamasaki A., et al. (2007) Mol. Biol. Cell 17:4876-4887
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_899195
Uniprot Accession Number
P55735
Species
Human
AA Sequence
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