Recombinant human VASP protein

Vasodilator-stimulated phosphoprotein, also known as VASP, is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. Recombinant human VASP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Vasodilator-stimulated phosphoprotein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.5 kDa (363aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

VASP, Vasodilator-stimulated phosphoprotein, ATGP1003-10 µg, ATGP1003-20 µg, ATGP1003-50 µg, ATGP1003-100 µg, ATGP1003-250 µg, ATGP1003-500 µg, ATGP1003-1 mg, ATGP1003-10, ATGP1003-20, ATGP1003-50, ATGP1003-100, ATGP1003-250, ATGP1003-500, ATGP1003-1

References & Citations

Laurent V., et al. (1999) J. Cell Biol. 144:1245-1258; ; Barzik M., et al. (2005) J. Biol. Chem. 280:28653-28662

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl, 0.1mM PMSF

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_003361

Uniprot Accession Number

P50552

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA QFAAGMASAL EALEGGGPPP PPALPTWSVP NGPSPEEVEQ QKRQQPGPSE HIERRVSNAG GPPAPPAGGP PPPPGPPPPP GPPPPPGLPP SGVPAAAHGA GGGPPPAPPL PAAQGPGGGG AGAPGLAAAI AGAKLRKVSK QEEASGGPTA PKAESGRSGG GGLMEEMNAM LARRRKATQV GEKTPKDESA NQEEPEARVP AQSESVRRPW EKNSTTLPRM KSSSSVTTSE TQPCTPSSSD YSD