Recombinant human RhoD protein

Rho-related GTP-binding protein, also known as RhoD, belongs to the small GTPase superfamily. The small GTPase Rho D contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution. Recombinant human RhoD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rho-related GTP-binding protein RhoD, ARHD, RHOHP1, RHOM, Ras homolog family member D, Rho-related protein HP1, RhoHP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.8 kDa (211aa) confirmed by MALDI-TOF

Additionnal Information

RhoD, Rho-related GTP-binding protein RhoD, ARHD, RHOHP1, RHOM, ATGP1002-10 µg, ATGP1002-20 µg, ATGP1002-50 µg, ATGP1002-100 µg, ATGP1002-250 µg, ATGP1002-500 µg, ATGP1002-1 mg, ATGP1002-10, ATGP1002-20, ATGP1002-50, ATGP1002-100, ATGP1002-250, ATGP1002-500, ATGP1002-1

References & Citations

Regnauld, K., et al. (2002) Oncogene. 21: 4020-4031.; ; Tsubakimoto., et al. (1999) Oncogene. 18: 2431-2440.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200 mM NaCl, 2mM EDTA, 0.1mM PMSF

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_055393

Uniprot Accession Number

O00212

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH DNVHAVFQEA AEVALSSRGR NFWRRITQGF C