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Recombinant human IDI2 protein

IDI2, also known as isopentenyl-diphosphate delta-isomerase 2, belongs to the IPP isomerase type 1 family. IDI2 catalyzes the 1, 3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP) . Recombinant human IDI2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Isopentenyl-diphosphate delta-isomerase 2, IPPI2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

28.9 kDa (247aa) confirmed by MALDI-TOF

Additionnal Information

IDI2, Isopentenyl-diphosphate delta-isomerase 2, IPPI2, ATGP1000-10 µg, ATGP1000-20 µg, ATGP1000-50 µg, ATGP1000-100 µg, ATGP1000-250 µg, ATGP1000-500 µg, ATGP1000-1 mg, ATGP1000-10, ATGP1000-20, ATGP1000-50, ATGP1000-100, ATGP1000-250, ATGP1000-500, ATGP1000-1

References & Citations

Clizbe D.B., et al. (2007) Biol Chem. 282:6668-6676; ; Ota T., et al. (2004) Genet. 36:40-45

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

NP_150286

Uniprot Accession Number

Q9BXS1

Species

Human

AA Sequence

MSDINLDWVD RRQLQRLEEM LIVVDENDKV IGADTKRNCH LNENIEKGLL HRAFSVVLFN TKNRILIQQR SDTKVTFPGY FTDSCSSHPL YNPAELEEKD AIGVRRAAQR RLQAELGIPG EQISPEDIVF MTIYHHKAKS DRIWGEHEIC YLLLVRKNVT LNPDPSETKS ILYLSQEELW ELLEREARGE VKVTPWLRTI AERFLYRWWP HLDDVTPFVE LHKIHRV
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