Recombinant human PIN4 protein

PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

18.8 kDa (176aa) confirmed by MALDI-TOF

Additionnal Information

PIN4, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17, ATGP0998-10 µg, ATGP0998-20 µg, ATGP0998-50 µg, ATGP0998-100 µg, ATGP0998-250 µg, ATGP0998-500 µg, ATGP0998-1 mg, ATGP0998-10, ATGP0998-20, ATGP0998-50, ATGP0998-100, ATGP0998-250, ATGP0998-500, ATGP0998-1

References & Citations

Uchida T., et al. (1999) FEBS Lett. 446:278-282; ; Fujiyama-Nakamura S., et al. (2009) Mol. Cell. Proteomics. 8:1552-1565

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

AAH93700

Uniprot Accession Number

Q9Y237

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK