Recombinant human LMP2/PSMB9 protein

PSMB9, also known as proteasome subunit beta type-9, is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This protein is located in the class II region of the MHC (major histocompatibility complex) . Expression of this protein is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Recombinant human PSMB9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Proteasome subunit beta type-9, LMP2, PSMB6i, RING12

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.5 kDa (220aa) confirmed by MALDI-TOF

Additionnal Information

LMP2, PSMB9, Proteasome subunit beta type-9, LMP2, PSMB6i, RING12, ATGP0997-10 µg, ATGP0997-20 µg, ATGP0997-50 µg, ATGP0997-100 µg, ATGP0997-250 µg, ATGP0997-500 µg, ATGP0997-1 mg, ATGP0997-10, ATGP0997-20, ATGP0997-50, ATGP0997-100, ATGP0997-250, ATGP0997-500, ATGP0997-1

References & Citations

Jia M., et al, (2010) Proteomics Clin Appl. 4 (10-11) :816-28 ; ; Kelly A., et al, (1991) Nature 353 (6345) :667-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 50 mM NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002791.1

Uniprot Accession Number

P28065

Species

Human

AA Sequence

TTIMAVEFD GGVVMGSDSR VSAGEAVVNR VFDKLSPLHE RIYCALSGS AADAQAVADMA AYQLELHGIE LEEPPLVLAA ANVVRNISYK YREDLSAHLM VAGWDQREGG QVYGTLGGML TRQPFAIGGS GSTFIYGYVD AAYKPGMSPE ECRRFTTDAI ALAMSRDGSS GGVIYLVTIT AAGVDHRVIL GNELPKFYDE