Recombinant human URM1 protein
URM1 is ubiquitin-related modifier 1 homolog protein that primarily functions in the post-translational modification of proteins by way of the urmylation pathway. In studies with Saccharomyces cerevisiae, it has been found that urm1 covalently binds to its E1 activating enzyme, uba4p, to conjugate alkyl hydroperoxide reductase (Ahp1) . It is hypothesized that this complex may then play a role in the oxidative-stress response in mammals. Recombinant human uRM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-related modifier 1 homolog, C9orf74, MGC2668, RP11-339B21.4
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
13.5 kDa (121aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Xu J., et al. (2006) Proc Natl Acad Sci u S A. 103 (31) :11625-30. ; ; Schlieker CD., et al. (2008) Proc Natl Acad Sci u S A. 105 (47) :18255-60.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_112176
Uniprot Accession Number
Q9BTM9
Species
Human
AA Sequence
MAAPLSVEVE FGGGAELLFD GIKKHRVTLP GQEEPWDIRN LLIWIKKNLL KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SVLFISTLHG G
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