Recombinant human TPM4 protein

TPM4 belongs to the tropomyosin family. Tropomyosins are present in virtually all eukaryotic cells (both muscle and nonmuscle), where they bind actin filaments and function to modulate actin-myosin interaction and stabilize actin filament structure. TPM4 binds to actin filaments in muscle and nonmuscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. Recombinant human TPM4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tropomyosin alpha-4 chain, Tropomyosin 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30.7 kDa (268aa) confirmed by MALDI-TOF

Additionnal Information

TPM4, Tropomyosin alpha-4 chain, Tropomyosin 4, ATGP0992-10 µg, ATGP0992-20 µg, ATGP0992-50 µg, ATGP0992-100 µg, ATGP0992-250 µg, ATGP0992-500 µg, ATGP0992-1 mg, ATGP0992-10, ATGP0992-20, ATGP0992-50, ATGP0992-100, ATGP0992-250, ATGP0992-500, ATGP0992-1

References & Citations

Lehman W., et al. (2000) J Mol Biol. 302 (3) :593-606. ; ; Wilton SD., et al. (1996) Cytogenet Cell Genet. 72 (4) :294-6.

Other References

Karasawa R, et al. Association of Anti-TPM4 autoantibodies with vasculopathic cutaneous manifestations in juvenile dermatomyositis. (Rheumatology (Oxford) . 2023) {https://pubmed.ncbi.nlm.nih.gov/37144941/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_003281

Uniprot Accession Number

P67936

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA ALNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK DEEKMEIQEM QLKEAKHIAE EADRKYEEVA RKLVILEGEL ERAEERAEVS ELKCGDLEEE LKNVTNNLKS LEAASEKYSE KEDKYEEEIK LLSDKLKEAE TRAEFAERTV AKLEKTIDDL EEKLAQAKEE NVGLHQTLDQ TLNELNCI