Recombinant human Calcineurin B2/PPP3R2 protein

PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein phosphatase 3 regulatory subunit B beta, Protein phosphatase 2B regulatory subunit 2, Calcineurin subunit B type 2 19kDa, Calcineurin B-like protein, CBLP, Calcineurin BII, CNBII, PPP3R1-like, PPP3RL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22 kDa (193aa) confirmed by MALDI-TOF

Additionnal Information

PPP3R2, Protein phosphatase 3 regulatory subunit B type 2, Protein phosphatase 3, regulatory subunit B type 2, PPP3RL, ATGP0989-10 µg, ATGP0989-20 µg, ATGP0989-50 µg, ATGP0989-100 µg, ATGP0989-250 µg, ATGP0989-500 µg, ATGP0989-1 mg, ATGP0989-10, ATGP0989-20, ATGP0989-50, ATGP0989-100, ATGP0989-250, ATGP0989-500, ATGP0989-1

References & Citations

Ueki K., et al. (1992) Biochem Biophys Res Commun. 187 (1) :537-43. ; ; Liu L., et al. (2005) Mol Biol Rep. 32 (1) :41-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_671709.1

Uniprot Accession Number

Q96LZ3

Species

Human

AA Sequence

MSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFS IYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV