Recombinant human ALKBH3 protein
ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. Recombinant human ALKBH3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3, DEPC-1, DEPC1, PCA1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.8 kDa (325aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ougland R., et al. (2004) Mol Cell. 16 (1) :107-16. ; ; Duncan T., et al. (2002) Proc Natl Acad Sci u S A. 99 (26) :16660-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_631917.1
Uniprot Accession Number
Q96Q83
Species
Human
AA Sequence
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