Recombinant human NECAP2 protein

NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s alpha-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis. Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NECAP endocytosis associated 2, FLJ10420

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30.5 kDa (283aa) confirmed by MALDI-TOF

Additionnal Information

NECAP2, NECAP endocytosis associated 2, FLJ10420, ATGP0973-10 µg, ATGP0973-20 µg, ATGP0973-50 µg, ATGP0973-100 µg, ATGP0973-250 µg, ATGP0973-500 µg, ATGP0973-1 mg, ATGP0973-10, ATGP0973-20, ATGP0973-50, ATGP0973-100, ATGP0973-250, ATGP0973-500, ATGP0973-1

References & Citations

Miller GJ., et al. (2003) Nat Struct Biol. 10 (8) :599-606. ; ; Mattera R., et al. (2004) J Biol Chem. 279 (9) :8018-28.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_060560

Uniprot Accession Number

Q9NVZ3

Species

Human

AA Sequence

MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF