Recombinant human eIF-3K/EIF3K protein
EIF3K, also known as eukaryotic translation initiation factor 3 subunit K, belongs to the eIF3 subunit K family. It is the smallest subunit of eIF3 and it interacts with several other subunits of eIF3 and the 40S ribosomal subunit. This protein is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. It is distributed both in nucleus and cytoplasm and colocalizes with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4) . Recombinant human EIF3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
27.2 kDa (238aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mayeur G.L., et al. (2003) . Eur. J. Biochem, 270: 4133-4139.; ; Wei Z., et al. (2004) . J. Biol. Chem. 279: 34983-34990.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_037366
Uniprot Accession Number
Q9UBQ5
Species
Human
AA Sequence
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