Recombinant human TREX2 protein

TREX2 is an enzyme containing 3-prime-to-5-prime exonuclease activity and removes mismatched, modified, fragmented, and normal nucleotides to generate the appropriate 3-prime termini for subsequent steps in the DNA metabolic pathways. This enzyme is involved in DNA replication, repair, and recombination. Recombinant human TREX2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Three prime repair exonuclease 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.0 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

TREX2, Three prime repair exonuclease 2, ATGP0970-10 µg, ATGP0970-20 µg, ATGP0970-50 µg, ATGP0970-100 µg, ATGP0970-250 µg, ATGP0970-500 µg, ATGP0970-1 mg, ATGP0970-10, ATGP0970-20, ATGP0970-50, ATGP0970-100, ATGP0970-250, ATGP0970-500, ATGP0970-1

References & Citations

Parra D., et al. (2009) Cancer Res. 69 (16) :6676-84.; ; Perrino FW., et al. (2008) J Biol Chem. 283 (31) :21441-52.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 30% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_542432

Uniprot Accession Number

Q9BQ50

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSEAPRAETF VFLDLEATGL PSVEPEIAEL SLFAVHRSSL ENPEHDESGA LVLPRVLDKL TLCMCPERPF TAKASEITGL SSEGLARCRK AGFDGAVVRT LQAFLSRQAG PICLVAHNGF DYDFPLLCAE LRRLGARLPR DTVCLDTLPA LRGLDRAHSH GTRARGRQGY SLGSLFHRYF RAEPSAAHSA EGDVHTLLLI FLHRAAELLA WADEQARGWA HIEPMYLPPD DPSLEA