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Recombinant human PFDN6 protein

PFDN6, also known as HKE2 or PFD6, is a subunit of the heteromeric prefoldin complex that chaperones nascent actin and alpha- and beta-tubulin chains pending their transfer to the cytosolic chaperonin containing TCP1 (CCT) complex. It binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it and binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Prefoldin subunit 6, H2-KE2, HKE2, KE-2, MGC70744, PFD6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.7 kDa (149aa) confirmed by MALDI-TOF

Additionnal Information

PFDN6, Prefoldin subunit 6, H2-KE2, HKE2, KE-2, MGC70744, PFD6, ATGP0966-10 µg, ATGP0966-20 µg, ATGP0966-50 µg, ATGP0966-100 µg, ATGP0966-250 µg, ATGP0966-500 µg, ATGP0966-1 mg, ATGP0966-10, ATGP0966-20, ATGP0966-50, ATGP0966-100, ATGP0966-250, ATGP0966-500, ATGP0966-1

References & Citations

Sowa ME., et al. (2009) Cell. 138 (2) :389-403.; ; Simons CT., et al. (2004) J Biol Chem. 279 (6) :4196-203.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_055075

Uniprot Accession Number

O15212

Species

Human

AA Sequence

MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA

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