Recombinant human HNRNPC protein

HNRNPC, also known as hnRNP C1/C2, belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Recombinant human HNRNPC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRNP, HNRPC, SNRPC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.5 kDa (313aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

HNRNPC, Heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRNP, HNRPC, SNRPC, ATGP0965-10 µg, ATGP0965-20 µg, ATGP0965-50 µg, ATGP0965-100 µg, ATGP0965-250 µg, ATGP0965-500 µg, ATGP0965-1 mg, ATGP0965-10, ATGP0965-20, ATGP0965-50, ATGP0965-100, ATGP0965-250, ATGP0965-500, ATGP0965-1

References & Citations

Hayakawa H., et al. (2010) Biochem Biophys Res Commun. 403 (2) :220-4.; ; Stone JR., et al. (2002) J Biol Chem. 277 (18) :15621-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 30% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004491

Uniprot Accession Number

P07910

Species

Human

AA Sequence

MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS AAEMYGSSFD LDYDFQRDYY DRMYSYPARV PPPPPIARAV VPSKRQRVSG NTSRRGKSGF NSKSGQRGSS KSGKLKGDDL QAIKKELTQI KQKVDSLLEN LEKIEKEQSK QAVEMKNDKS EEEQSSSSVK KDETNVKMES EGGADDSAEE GDLLDDDDNE DRGDDQLELI KDDEKEAEEG EDDRDSANGE DDS