Recombinant human c-Jun protein
The human protooncogene c-Jun is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. c-Jun and c-Fos form a complex in the nucleus. AP1 is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21, p19 and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. Recombinant human c-Jun protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Transcription factor AP-1, AP-1, AP1, c-Jun, jun proto-oncogene
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.3 kDa (261aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bohmann D., et al. (1987) Science. 238 (4832) :1386-92. ; ; Bannister AJ., et al. (1993) Nucleic Acids Res. 21 (5) :1289-95.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Scientific Category
Cancer
NCBI Accession Number
NP_002219
Uniprot Accession Number
P05412
Species
Human
AA Sequence
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