Recombinant human A2LD1/GGACT protein

A2LD1, also known as gamma-glutamylaminecyclotransferase, is an enzyme that converts gamma-glutamylamines to free amines and 5-oxoproline. It shows high activity toward gamma-glutamyl-epsilon-lysine, derived from the breakdown of fibrin and other proteins cross-linked by transglutaminases. This protein adopts the newly identified cyclotransferase fold, observed in gamma-glutamylcyclotransferase, an enzyme with activity toward gamma-glutamyl-alpha-amino acids. Recombinant human A2LD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Gamma-glutamylaminecyclotransferase, GGACT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.4 kDa (173aa) confirmed by MALDI-TOF

Additionnal Information

A2LD1, Gamma-glutamylaminecyclotransferase, Gamma glutamylaminecyclotransferase, GGACT, , ATGP0959-10 µg, ATGP0959-20 µg, ATGP0959-50 µg, ATGP0959-100 µg, ATGP0959-250 µg, ATGP0959-500 µg, ATGP0959-1 mg, ATGP0959-010, ATGP0959-020, ATGP0959-050, ATGP0959-100, ATGP0959-250, ATGP0959-500, ATGP0959-01M

References & Citations

Oakley AJ, ., et al. (2010), J Biol Chem., 285:9642-8.; ; Danson JW., et al. (2002), Anal Biochem., 303:120-30.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_149101

Uniprot Accession Number

Q9BVM4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MALVFVYGTL KRGQPNHRVL RDGAHGSAAF RARGRTLEPY PLVIAGEHNI PWLLHLPGSG RLVEGEVYAV DERMLRFLDD FESCPALYQR TVLRVQLLED RAPGAEEPPA PTAVQCFVYS RATFPPEWAQ LPHHDSYDSE GPHGLRYNPR ENR