Recombinant human EF-1 delta/EEF1D protein

Eukaryotic translation elongation factor 1 delta, also known as EEF1D, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Overexpression of EEF1D is associated with oesophageal carcinoma and may adversely affect the outcome of medulloblastomas. Recombinant human EEF1D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation elongation factor 1 delta, EF-1D, EF1D, Elongation factor 1-delta, Antigen NY-CO-4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.2 kDa (301aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

EEF1D, Eukaryotic translation elongation factor 1 delta, EF-1D, EF1D, ATGP0955-10 µg, ATGP0955-20 µg, ATGP0955-50 µg, ATGP0955-100 µg, ATGP0955-250 µg, ATGP0955-500 µg, ATGP0955-1 mg, ATGP0955-10, ATGP0955-20, ATGP0955-50, ATGP0955-100, ATGP0955-250, ATGP0955-500, ATGP0955-1

References & Citations

De Bortoli M., et al. (2006) BMC Cancer. 6:223.; ; Cans C., et al. (2003) Proc Natl Acad Sci. 100:13892-13897.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001951

Uniprot Accession Number

P29692

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I