Recombinant human BAG2 protein

BCL2-associated athanogene 2, also known as BAG2, is a member of the Bag family of proteins. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain. BAG2 is a major component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex and acts to disrupt CHIP-mediated ubiquitylation. Recombinant human BAG2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

BCL2-associated athanogene 2, BAG-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.9 kDa (231aa) confirmed by MALDI-TOF

Additionnal Information

BAG2, BCL2-associated athanogene 2, BAG-2, ATGP0952-10 µg, ATGP0952-20 µg, ATGP0952-50 µg, ATGP0952-100 µg, ATGP0952-250 µg, ATGP0952-500 µg, ATGP0952-1 mg, ATGP0952-10, ATGP0952-20, ATGP0952-50, ATGP0952-100, ATGP0952-250, ATGP0952-500, ATGP0952-1

References & Citations

Takayama S., et al. (1999) J Biol Chem. 274:781-786.; ; Arndt V., et al. (2005) CHIP Mol Biol Cell. 16:5891-5900.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_004273.1

Uniprot Accession Number

O95816

Species

Human

AA Sequence

MAQAKINAKA NEGRFCRSSS MADRSSRLLE SLDQLELRVE ALREAATAVE QEKEILLEMI HSIQNSQDMR QISDGEREEL NLTANRLMGR TLTVEVSVET IRNPQQQESL KHATRIIDEV VNKFLDDLGN AKSHLMSLYS ACSSEVPHGP VDQKFQSIVI GCALEDQKKI KRRLETLLRN IENSDKAIKL LEHSKGAGSK TLQQNAESRF N