Recombinant human KDSR protein

KDSR, also known as 3-ketodihydrosphingosine reductase, is a 332 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum (ER) and belongs to the short-chain dehydrogenases/reductases (SDR) family. KDSR is a secreted protein which is weakly expressed in hematopoietic tissue. Also this protein catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS) Recombinant human KDSR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

3-ketodihydrosphingosine reductase, DHSR, FVT1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.0 kDa (266aa) confirmed by MALDI-TOF

Additionnal Information

KDSR, 3-ketodihydrosphingosine reductase, DHSR, FVT1, ATGP0948-10 µg, ATGP0948-20 µg, ATGP0948-50 µg, ATGP0948-100 µg, ATGP0948-250 µg, ATGP0948-500 µg, ATGP0948-1 mg, ATGP0948-10, ATGP0948-20, ATGP0948-50, ATGP0948-100, ATGP0948-250, ATGP0948-500, ATGP0948-1

References & Citations

Kihara A., et al. (2004) J. Biol. Chem. 279:49243-49250; ; Parkinson, N.J., et al. (2008) Neuromuscul. Disord. 18: 394-397.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

NP_002026

Uniprot Accession Number

Q06136

Species

Human

AA Sequence

KPLALPGAH VVVTGGSSGI GKCIAIECYK QGAFITLVAR NEDKLLQAKK EIEMHSINDK QVVLCISVDV SQDYNQVENV IKQAQEKLGP VDMLVNCAGM AVSGKFEDLE VSTFERLMSI NYLGSVYPSR AVITTMKERR VGRIVFVSSQ AGQLGLFGFT AYSASKFAIR GLAEALQMEV KPYNVYITVA YPPDTDTPGF AEENRTKPLE TRLISETTSV CKPEQVAKQI VKDAIQGNFN SSLGSD